禽呼肠孤病毒感染对SPF鸡外周血淋巴细胞中天然免疫相关基因转录的影响

doi:10.11843/j.issn.0366-6964.2017.01.014

摘要/Abstract

摘要：

为了明确禽呼肠孤病毒(avian reovirus, ARV)感染对宿主天然免疫应答的影响，利用实时荧光定量PCR方法检测ARV感染SPF鸡后外周血淋巴细胞中天然免疫相关基因，包括Toll样受体（TLRs）、MDA5、IPS-1、IRF-3、IFN-α、IFN-β、IFN-γ、IFITM3、Mx1和OASL等基因在感染后0、12 h以及1、2、3、5和7 d的转录水平变化。结果表明， ARV感染SPF鸡后7 d以内，外周血淋巴细胞中TLR3和TLR21的mRNA转录水平显著降低（P<0.05或P<0.01）；而TLR7和MDA5的mRNA转录水平则在整个试验过程中显著升高，分别在7 dpi和1 dpi达到峰值（P<0.05或P<0.01）。IPS-1的mRNA转录被抑制；IRF-3则呈显著转录上调趋势；IFN-α、IFN-β和IFN-γ均表达上调，分别在2~3 dpi达到峰值（P<0.05或P<0.01）。IFITM3、Mx1、OASL的mRNA转录水平也显著升高，均在3 dpi达到峰值（P < 0.01）。试验结果表明ARV在感染早期能够显著性引起TLR7、MDA5、IRF3、IFN-α、IFN-β、IFN-γ、IFITM3、Mx1和OASL的转录上调，抑制TLR3、TLR21和IPS-1的转录，为进一步阐释ARV的分子致病机制和免疫应答机制奠定了基础。

Abstract:

This study was aimed to assess the influences of avian reovirus (ARV) infection on expression of innate immune genes in peripheral blood lymphocytes of SPF chickens.The quantitative real-time PCR assays was performed to determine the transcriptional levels of TLR3, TLR7, TLR21, MDA5, IPS-1, IRF-3, IFN-α, IFN-β, IFN-γ, IFITM3, Mx1 and OASL in 0 h, 12 h, 1, 2, 3, 5 and 7 days post infection (dpi) in peripheral blood lymphocytes of SPF chickens infected with ARV S1133. These results showed that the mRNA transcriptional levels of TLR3 and TLR21 were significantly reduced within 7 dpi （P<0.05 or P<0.01）; the mRNA transcriptional levels of TLR7 and MDA5 were obviously promoted and specially reached the summit maximum values on 7 dpi and 1 dpi, respectively (P<0.05 or P<0.01). The mRNA level of IPS-1 was down-regulated within 7 dpi. Meanwhile, the mRNA level of IRF-3 was up-regulated during the whole transcrption course, and IFN-α, IFN-β and IFN-γ mRNA levels were significantly increased and reached the peak at 2-3 dpi. The mRNA levels of IFITM3, Mx1 and OASL were significantly up-regulated and peaked at 3 dpi (P<0.01). Therefore, ARV infection is able to modulate the increased mRNA transcription of TLR7, MDA5, IRF-3, IFN-α, IFN-β, IFN-γ, IFITM3, Mx1 and OASL, and reduce the mRNA transcription of TLR3, TLR21 and IPS-1, which would contribute to further understand ARV pathogenesis and host innate immune responses to ARV infection.

中图分类号:

S852.659.4

黄莉,谢芝勋，蓝元喜，谢丽基，邓显文，范晴，罗思思，谢志勤，黄娇玲，张艳芳，王盛，曾婷婷. 禽呼肠孤病毒感染对SPF鸡外周血淋巴细胞中天然免疫相关基因转录的影响[J]. 畜牧兽医学报, 2017, 48(1): 116-123.

HUANG Li,XIE Zhi-xun, LAN Yuan-xi,XIE Li-ji, DENG Xian-wen, FAN Qing, LUO Si-si, XIE Zhi-qin, HUANG Jiao-ling, ZHANG Yan-fang, WANG Sheng, ZENG Ting-ting. Effects of Avian Reovirus Infection on Transcription of Innate Immune Genes in Peripheral Blood Lymphocytes of SPF Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 116-123.

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https://www.xmsyxb.com/CN/Y2017/V48/I1/116

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